The mechanism of action of restriction enzymes

2021.09.17 08:52 LeadingBiology The mechanism of action of restriction enzymes

The restriction enzymes can act on a specific sequence by recognizing a recognition sequence that is specific in its base sequence. the restriction enzyme produces a double-stranded cut. The recognition sequences can be classified based on the number of bases it has, the number of bases usually varies between 4 and 8 bases, and this determines this sequence will appear in the genome e.g., a 4-base pair sequence would theoretically occur once every 44 or 256bp. This sequence is palindromic, which means that it reads the same either way. Theoretically, there are two types of palindromic sequences present in DNA.
1)The mirror-like palindrome is similar to those which are found in a way where the sequence reads similar as forward and backward. e.g. GTA ATG. 2)The inverted repeat palindrome still reads the same forward and backward, but in complementary DNA strands (i.e., double-stranded DNA) as in GTATAC, the forward and backward sequences are found (GTATAC being complementary to CATATG). Inverted repeat palindromes are more frequent and biologically more important than mirror-like palindromes.
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2021.09.17 08:52 JSBFIFA New FIFA Content Creators - We all know Danny Aarons, Castro and runthefutmarket, but are there any lesser known creators who deserve a chance? Let me know my dudes

New FIFA Content Creators - We all know Danny Aarons, Castro and runthefutmarket, but are there any lesser known creators who deserve a chance? Let me know my dudes submitted by JSBFIFA to fut [link] [comments]


2021.09.17 08:52 prony1237 Can I get my first covid vaccine outside the United States and get my second while inside the USA?

Im currently in Latin America and have an opportunity to get my first shot. CAn I complete the vaccination when I return home in a couple months? Or will they turn a foreign card away.
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2021.09.17 08:52 calibuildr Carolyn Martin Swing Band

Carolyn Martin Swing Band submitted by calibuildr to Ameripolitan [link] [comments]


2021.09.17 08:52 Kwsagitarii Should I make a guild

My friends and I are thinking of making a small guild but I'm not sure if it will be worth it given the bonuses that a guild can give. I haven't played swtor in a while so could someone give some advice?
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2021.09.17 08:52 YoureStupidGoAway Need help identifying the type of sword (maybe dagger?) and hopefully the manufacturer! (More info in comments)

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2021.09.17 08:52 57nightjars I can't move

so, like, it looks like I moving, but I am not, everything else is, I flipped this little switch in my brain one day month ago, while thinking about things and like all of the horrible nightmare switches, I can't turn it off. so I can't move. I can try to move, but I don't. no one can move, and I can't tell the difference between walking in place and actually walking, cause they are the same thing. and walking makes me carsick now and this has lasted months and I am deeper in the labrinth now than before ever and I want all of this, both this and the other things to stop but they can't cause they are real, and, logically, why would I want to make myself incapable of noticing and handling these many threats to my life that I have to deal with, and I think I know how I look when I talk about this stuff, but maybe not, I am self-aware but in the "knowing you are on a plane that's about to crash and you can't do anything about it" way and not in the "being a pilot" way, which is the bad way cause I know what people think and I know that I am right, but I can't get out of the twisty brain spiderweb to deal with either of those things, and I did not explain that well cause I can't and still make sense, and I need to do things, but I can't and everyone can read my mind, and there are bigger things, and even the people I really trusted betrayed me a little, none of them are reliable, none of them can be trusted to care about my well-being, and maybe I don't know what people think, idk. also, you are probably in on it too, caus eyou all are, and that's not very considerate, and I think you should stop. maybe some of you aren't but I have yet to meet someone who didn't read my mind the first moment they got a chance, so, it must be rare. maybe you are all the same person, actually, and maybe I am that same person too, who knows? it makes sense. I like to think that maybe I won't die like a normal person, instead I will just think myself into too tight a spiral and I will be absorbed into the universe. I am not a person, of course, I am a robot, sorry if that breaks the rules, but I think most people disagree with me on that, and robots have feelings too, so I am probably allowed. but I think if you like, cut me open you wouldn't see human flesh. one time I time travled to the future, that was weird. now people time travel to me. my life is weird, and I wish it wasn't but I don't wish it wasn't I just kind of don't want my life to is, you know? I wish I could just be like, mourned, I can't feel human love, of course, but none of the people who would feel sad if I died know that, so they would. they wouldn't if they knew the truth, but they aare incapable of knowing the truth. oh, to be external. anyways, I can't be loved like a human, I can't love like a human, maybe people can be deluded into thinking I can be, you know? maybe that is the closest thing, and I won't die, but I wish I had a soft place to rest.
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2021.09.17 08:52 MeysemH Antons burberry QC

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2021.09.17 08:52 GiorgioPerlasca Contenuto per gli abbonati

Contenuto per gli abbonati submitted by GiorgioPerlasca to cazzeggio [link] [comments]


2021.09.17 08:52 Arthur_Boo_Radley U Hrvatskoj osnovan Sindikat radnika digitalnih platformi

U Hrvatskoj osnovan Sindikat radnika digitalnih platformi submitted by Arthur_Boo_Radley to dnevnik [link] [comments]


2021.09.17 08:52 Anne--Frankly How to get lineman to actually block?

Everyone say pass rush is non existent but my lines all 87+ and somehow never have blocked a dude yet. Granted I’m going against major money squads but I’d expect a block every now and then
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2021.09.17 08:52 lalablahblahhaha Are the Pen+Gear Pentel P207 knock offs the same quality and parts as an authentic Pentel?

I found this post
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2021.09.17 08:52 FryBoyter Get where you’re going faster, with Firefox Suggest

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2021.09.17 08:52 Hdcoach15 [PSN][H] List of items below [W] Credits or trade offers

these are all the items I have, comment what you are interested in
**Bodies:**
- Octane, Titanium White
- Harbinger GXT, cobalt
- R3MX, Cobalt, Aviator
- Tyranno GXT, Cobalt
- Tyranno GXT, Crimson
- Tyranno GXT, Lime
**Decals:**
- Aftershock:
- clipper
- Breakout:
- mammoth
- nitro circus
- dragon
- Dominus:
- gentleman beef
- Espee:
- eye opener
- Gizmo:
- wannabee
-Harbinger:
- quickline
-Merc:
- dragon
- narwhal
- watermelon
- Octane:
- Sarp stripe
-Peregrine TT:
- Mechwing
- Road Hog:
- watermelon
- snakeskin
-Ripper:
- stibium
- Universal:
- Auger, purple
- liquid camp, Victor, gray
- EQ-RL, lime
- Spatiotemporal, sky blue, Sniper
- Spatiotemporal, orange
- Spatiotemporal, purple
- Spatiotemporal, Titanium White
**Finishes**
- none currently
**Wheels:**
- 3-lobe, burnt sienna, goalkeeper
- 3-lobe, TW, guardian
- 3-lobe, cobalt, show-off
- Ault-spl, Crimson
- Ault-spl, forest green
- Ault-spl, lime
- DYR II, lime
- DYR II, pink
- Esoto 4R, black
- Esoto 4R inverted, Grey, guardian
- Esoto 4R inverted, sky blue, Sniper
- Ferris, grey
- Hamster, show-off, sky blue
- Hamster, burnt sienna
- Hamster, Grey
- Hamster, Crimson
- Hamster holo, lime, juggler
- Hamster holo, purple, juggler
- Hamster holo, forest green
- Hamster holo, orange
- HRB-20, Grey
- HRB-20, Purple
- Imptekk, forest green
- Imptekk, Grey
- Imptekk, saffron
- Joko XL, burnt sienna
- Joko XL, cobalt
- Joko XL, pink
- K2
- Mandala, TW
- Maxle-PA, pink
- Maxle-PA, saffron
- Maxle-PA, Titanium White
- NeYoYo
- Photon
- Polygeric, orange
- Polygeric, pink
- Polygerc inverted, pink
- Polygeric inverted, sky blue
- Propeller, saffron
- Propeller inverted, Crimson
- SARPBC
- Sk8ter, forest green
- Sk8ter, TW, Snipee
- SPN, Saffron
- SPN, sky blue
- String theory, cobalt
- String theory, orange
- String theory, sky blue
- Traction: Hatch, black
- Traction: Hatch, Crimson
- Traction: Hatch, saffron
- Woofer, Crimson
- Zadeh, burnt sienna
- Zadeh, purple
- Zadeh, Titanium White
- Zadeh S3 inverted, orange
- Zadeh S3 inverted, purple
- Zadeh S3 inverted, saffron
- Zefram
**Boost:**
- Chequered Flag, Crimson
- Chequered flag, pink
- Chequered flag, sky blue
- Diecast spark, black
- Diecast spark, Crimson
- Diecast spark, forest green
- ectoplasm
- Fireworks
- grass
- Monsoon, burnt sienna
- Monsoon, purple
- Monsoon, Titanium White
- Mood slime
- polygonal
- popcorn
- Pylon, burnt sienna
- Pylon, cobalt
- Pylon, lime
- Star Princess, burnt sienna
- Star Princess, orange
- Star Princess, pink
- Supernova, sky blue
- Supernova, black, show-off
- Supernova, Grey
- Supernova, Pink
- Toon flame, orange
- Toon flame, purple
**Topper:**
- None currently
**Antennas**
- Calaveras
- Nitro circus
**Goal explosion:**
- Chequered flag, Crimson
- Chequered flag, forest green
- Chequered flag, Titanium White
- Juiced
- Riser, purple, guardian
- Riser, black
- Riser, cobalt
- Spacial rift, lime
- Spatial rift, sky blue
- Mister Monsoon, sky blue, Tactition
**Trails:**
- Pixel pointer, Crimson
- Pixel pointer, purple
- Pixel pointer, Titanium White
**Banners:**
- Hornet
- Wet wash
submitted by Hdcoach15 to RocketLeagueExchange [link] [comments]


2021.09.17 08:52 Least2020-2022 Train Tickets Available Tomorrow: Ready for National Holiday

Train Tickets Available Tomorrow: Ready for National Holiday submitted by Least2020-2022 to ReferChina [link] [comments]


2021.09.17 08:52 Micro_Mimi Did the coronavirus jump from animals to people twice?

Did the coronavirus jump from animals to people twice? submitted by Micro_Mimi to Coronavirus_Ireland [link] [comments]


2021.09.17 08:52 Shardonnay89 πŸŽ–β˜‘οΈπŸ’±πŸ‘¨πŸΎβ€βš–οΈ #SafeMoonArmy Updated Exchange Information. We discovered Hotbit has auto staking, rewarding daily if you hold. They did it on the DL, people just started reporting they were getting rewards there.

πŸŽ–β˜‘οΈπŸ’±πŸ‘¨πŸΎβ€βš–οΈ #SafeMoonArmy Updated Exchange Information. We discovered Hotbit has auto staking, rewarding daily if you hold. They did it on the DL, people just started reporting they were getting rewards there. submitted by Shardonnay89 to SafeMoon [link] [comments]


2021.09.17 08:52 Chrontius Ventenna is shutting down

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2021.09.17 08:52 vitiligora Do you need to light up ?

Do you need to light up ? submitted by vitiligora to smokingfetishcreators [link] [comments]


2021.09.17 08:52 Chari2ar3 Can you not run after getting shot by tagilla?

Couldn't run after getting shot in the arm once
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2021.09.17 08:52 chickennopasta stages of grief

stages of grief submitted by chickennopasta to EpicSeven [link] [comments]


2021.09.17 08:52 Johannlaji0902 Eight Future Middle-Order Options For India In Tests After Pujara, Kohli And Rahane Move On

Eight Future Middle-Order Options For India In Tests After Pujara, Kohli And Rahane Move On submitted by Johannlaji0902 to Cricket [link] [comments]


2021.09.17 08:52 Azhar-MS Level two in feb

For those who passed level one of July.when are planning to take level 2? Am thinking of feb but am afraid it’s a little bit aggressive! Please advice
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2021.09.17 08:52 Flashy-Description68 Assessment of our ethical ETF portfolio?

We started investing in ETFs last year and are just slowly learning. We're about to add some more significant numbers into our portfolio, so I was wondering if anyone could provide some feedback as to our (target) portfolio.
We are in our 30s with a minimum 10-year investment horizon and focused on growth, so are willing to take some risk.
We are also not too adverse to adding DHHF or VDHG if people say that be better for diversification and potentially growth (I know no one has a crystal ball).
ETHI 65% FAIR 20% IMPQ 10% GBND 5%
I like ETHI but has does it have too much exposure to tech?
IMPQ we know is expensive but we liked that it's small and medium caps and is actively managed and uses its proceeds to make a difference. Does its 8% cash holdings seem quite conservative?
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2021.09.17 08:52 tyler1118 The Tennessee Titans were MANHANDLED by The Arizona Cardinals DL - Reviewed by Former NFL OL Uche Nwaneri.

The Tennessee Titans were MANHANDLED by The Arizona Cardinals DL - Reviewed by Former NFL OL Uche Nwaneri. submitted by tyler1118 to AZCardinals [link] [comments]


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